Jonathan Dworkin, Ph.D.
We are interested in how various bacteria (e.g., the model Gram+ spore-former B. subtilis
and S. aureus
) enter in dormancy and also re-initiate growth. We have identified several well-conserved, eukaryotic-like Ser/Thr kinase that are essential for these processes and are investigating the mechanism underlying their function. Among their targets are two essential GTPases, EF-Tu and EF-G, that are key in regulating protein synthesis. Specifically, we are examining how this phosphorylation changes the activity of these protein. In addition, we are examining the role of this kinase in antibiotic resistance and survival during stationary phase.
The lab is also interested in peptidoglycan synthesis and we are focusing on how this essential process is regulated. We have identified a key role of a kinase and are investigating the mechanistic basis for this regulation.
- Libby, E.A. and Dworkin, J. (2017) Habits of highly effective biofilms: Ion signaling. Molecular Cell 66: 1.
- Sarkar, S., Libby, E.A., Pidgeon, S.E., Dworkin, J. and Pires, M.M. (2016) In vivo probe of Lipid II-interacting proteins. Angewandte Chemie doi: 10.1002/anie.201603441.
- Sturm, A. and Dworkin, J. (2015) Phenotypic diversity as a mechanism to exit dormancy. Current Biology 25: 1-6. (Cited in Dispatch, Current Biology 25: 45-47.)
- Libby, E.A., Goss, L.A. and Dworkin, J. (2015) The eukaryotic-like Ser/Thr kinase PrkC regulates the essential WalRK two-component system in Bacillus subtilis. PLoS Genetics 11: e1005275.
- Pereira, S.P.P., Gonzalez, R. and Dworkin, J. (2015) Protein synthesis during cellular quiescence is inhibited by phosphorylation of a translational elongation factor. Proc. Natl. Acad. Sci. U.S.A. 112: e3274-3281.
- Dworkin, J. (2015) Ser/Thr phosphorylation as a regulatory mechanism in bacteria. Curr. Opin. Microbiol. 24C: 47-52.
- Dworkin, J. (2014) The medium is the message: interspecies and interkingdom signaling by peptidoglycan and related bacterial glycans. Annu. Rev. Microbiol. 68: 137-154.
- Dworkin, J. (2014) Protein targeting during sporulation. In The Bacterial Spore: From Molecules to Systems, Driks, A. and Eichenberger, P., Eds. ASM Press.
- Squeglia, F., Marchetti, R., Ruggiero, A., Lanzetta, R., Marasco, D., Dworkin, J., Pethoukov, M., Molinaro, A., Berisio, R. and Silipo, A. (2012) Chemical basis of peptidoglycan discrimination by PrkC, a key kinase Involved in bacterial resuscitation from dormancy. J. Amer. Chem. Soc. 133: 20676-20679.
- Higgins D, Dworkin J. (2012) Recent progress in Bacillus subtilis sporulation. FEMS Microbiol Rev. 36: 131-148.
- Laaberki, M-H., Pfeffer, J., Clarke, A. and Dworkin J. (2011) O-acetylation of peptidoglycan is required for proper cell separation and S-layer anchoring in Bacillus anthracis. J. Biol Chem 286: 5278-5288.
- Pereira, S., Goss, L., and Dworkin, J. (2011) Eukaryote-like Ser/Thr kinases and phosphatases in bacteria. Microbiology and Molecular Biology Reviews 75: 1-21.
- Dworkin, J. and Shah, I. (2010) Exit from dormancy in microbial organisms. Nature Reviews Microbiology 8: 890-896.
- Dworkin, J. (2010) Form equals function? Bacterial shape and its consequences for pathogenesis. Mol. Microbiol. 78: 792-795.
- Fay, A., Meyer, P. and Dworkin, J. (2010) Interactions between late-acting proteins required for peptidoglycan synthesis in sporulation. J. Mol. Biol. 399: 547-561.
- Meyer, P., Gutierez, J., Pogliano, K. and Dworkin, J. (2010) Cell wall synthesis is necessary for membrane dynamics during sporulation of Bacillus subtilis. Mol. Microbiol. 76: 956-970.
- Shah, I.M. and Dworkin, J. (2010) Induction and regulation of a secreted peptidoglycan hydrolase by a membrane Ser/Thr kinase that detects muropeptides. Mol. Microbiol. 75: 1232-1243.
- Fay, A. and Dworkin, J. (2009) B. subtilis homologs of the putative Lipid II flippase E. coli MurJ (MviN) are not essential. J. Bacteriol. 191: 6020-6028. (Cited by Faculty of 1000)
- Eldar, A., Chary, V., Xenopoulos, P., Fontes, M.E., Loson, O.C., Dworkin, J., Piggot, P. and Elowitz, M. (2009) Partial penetrance facilitates developmental evolution in bacteria. Nature 460: 510-515.
- Dworkin, J. (2009) Cellular polarity in prokaryotic organisms. Cold Spring Harb. Perspect. Biol. 1: a003368.
- Shah, I., and Dworkin J. (2009) Dispatch: Bacteria talk to (some) of their neighbors. Current Biology 19: R689-691.
- Shah, I.M., Laaberki, M-H, Popham, D.L. Dworkin, J. (2008) A eukaryotic-like Ser/Thr kinase signals bacteria to exit dormancy in response to peptidoglycan fragments. Cell 135: 486-497.
- Laaberki, M-H. and Dworkin, J. (2008) The role of spore coat proteins in the resistance of B. subtilis spores to C. elegans predation. J. Bacteriol. 190: 6197-6204.
- Laaberki, M-H. and Dworkin, J. (2008) Death and survival of spore-forming bacteria in the Caenorhabditis elegans intestine. Symbiosis 46: 195-200.
- Real, G., Fay, A., Eldar, A., Pinto, S.M., Henriques, A.O. and Dworkin, J. (2008) Determinants for the subcellular localization and function of a non-essential SEDS protein. J. Bacteriol. 190: 363-376.
- Suel, G., Kulkarni, R.P., Dworkin, J., Garcia-Ojalvo, J. and Elowitz, M. (2007) Tunability and noise dependence in differentiation dynamics. Science 315: 316-9.
- Meyer, P. and Dworkin, J. (2007) Applications of fluorescence microscopy to single bacterial cells. Res. Microbio. 158: 187-194.
- Dworkin, J. and Losick, R. (2005) Developmental committment in a bacterium. Cell 121: 401-409.
- Dworkin J. (2003) Transient genetic asymmetry and cell fate in a bacterium. Trends in Genetics 19: 107-112.
- Dworkin J. and Losick R. (2002) Does RNA polymerase help drive chromosome segregation in bacteria? Proc. Natl. Acad. Sci. U.S.A. 99: 14089-14094.
- Dworkin J. and Losick R. (2001) Differential gene expression governed by chromosomal spatial asymmetry. Cell 107: 339-346.
- Dworkin J. and Losick R. (2001) Linking nutritional status to gene activation and development. Genes Dev. 15: 1051-1055.
Associate Professor Jonathan DworkinPhone: